Mmp 9 Antibody Cell Signaling

2001 curr opin cell biol 13 534 40.

Mmp 9 antibody cell signaling.

Once synthesized mmp exists as a latent proenzyme. Incubate with rotation overnight at 4 c. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding phe542 of human mmp 9 protein. Add protein a agarose 10 30 µl of 50 bead slurry.

Antibodies are purified by protein a and peptide affinity chromatography. Incubate with rotation for 1 3 hr at 4 c. Maximum mmp activity requires proteolytic cleavage to generate active mmps by releasing the inhibitory propeptide domain from the full length protein 5. Matrix metalloproteinase 9 92 kda gelatinase 92 kda type iv collagenase gelatinase b mmp9 human.

Western blot analysis was performed using mmp 2 d4m2n rabbit mab as primary antibody. Background the matrix metalloproteinases mmps are a family of proteases that target many extracellular proteins including other proteases growth factors cell surface receptors and adhesion. Add primary antibody at the appropriate dilution as recommended in the product datasheet to 200 µl cell lysate at 1 mg ml. Antibodies are purified by protein a and peptide affinity chromatography.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence based around amino acid residue gly657 of human mmp 9. Mmp 9 is a matrix metalloprotease produced from immune cells including macrophages and neutrophils which plays important roles in wound healing immunity and angiogenesis. Anti rabbit igg hrp linked antibody 7074 was used as secondary antibody. Lane 1 is 10 input lane 2 is rabbit da1e mab igg xp isotype control 3900 and lane 3 is mmp 2 d4m2n rabbit mab.

Background the matrix metalloproteinases mmps are a family of proteases that target many extracellular proteins including other proteases growth factors cell surface receptors and adhesion. It was detected by western blot in total lysate of human primary leukemia blasts. Microcentrifuge for 30 sec at 4 c. Mmp 13 is synthesized as a latent proenzyme and proteolytic removal of the inhibitory propeptide domain is required for enzyme activation.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lys115 of human mmp 9. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lys554 of human mmp 2 protein. In this product review i report results that the anti mouse mmp 9 antibody from cell signaling showed unsurpassed performance in detecting the protein in both western blotting and immunofluroscence microscopy. Mmp 13 is a member of a subgroup of collagenases including mmp 1 mmp 8 and mmp 18 that play an even more important function targeting fibrillar collagen.

Immunoprecipitation of mmp 2 protein from u 118mg cell extracts.